Plasmid DNA for packaging AAV; Recombinant AAV (stereotypes 1-9, retro, PhP.eb, PhPs); primary antibodies to Brainbow FPs;
Description of what it is used for
Labeling Cre expressing cells stochastically in colors
Description of its capabilities
Allow labeling the fine neuronal structures of neighboring neurons with membrane targeted Brainbow FPs
Location (Research Facility)
Plasmid DNA [Addgene]; AAVs [University of Michigan Vector Core]; Brainbow FP antibody [kerafast]
Link to a User Manual
Roossien DH, Cai D; Methods in Molecular Biology 2017;1642:211-228
Type of research that was enhanced by its use
Single and multiple neuron morphology reconstruction in densely labeled samples