Magnetically sensitive ion channels would allow researchers to better study how specific brain cells affect behavior in freely moving animals; however, recent reports of “magnetogenetic” ion channels based on biogenic ferritin nanoparticles have been questioned.
Studies of amnesic patients and animal models support a systems consolidation model, which posits that explicit memories formed in hippocampus are transferred to cortex over time. Prelimbic cortex is required for the expression of learned fear memories from hours after learning until weeks later.
Categorically distinct basic drives can exert potent influences on each other; such interactions are likely to have important adaptive consequences and can become maladaptive.
Multicolor labeling of cardiac muscle with systemic AAVs.
Trial-to-trial variability is a reflection of the circuitry and cellular physiology that make up a neuronal network. A pervasive yet puzzling feature of cortical circuits is that despite their complex wiring, population-wide shared spiking variability is low dimensional.
Conference on Lasers and Electro-Optics, OSA Technical Digest (online)
We demonstrate an adaptive femtosecond laser source that improves the imaging speed by >10 times for multiphoton imaging of brain activity in awake mouse, achieving 30 frames/s, 734×734 µm field-of-view (FOV) at 700 µm depth.
Interferometric spatial frequency modulation for imaging (I-SPIFI) is demonstrated for the first time, to our knowledge. Significantly, this imaging modality can be seamlessly combined with nonlinear SPIFI imaging and operates through single-element detection.
Neuroscience is experiencing a revolution in which simultaneous recording of thousands of neurons is revealing population dynamics that are not apparent from single-neuron responses. Deeper understanding of this structure requires studying phenomena detected in single trials, which is challenging.
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation.
How does attentional modulation of neural activity enhance performance? Here we use a deep convolutional neural network as a large-scale model of the visual system to address this question.
The naturally occurring channelrhodopsin variant anion channelrhodopsin-1 (ACR1) exhibits large light-gated anion conductance and high anion selectivity when expressed in heterologous settings.
Biomedical Optics Express
Light attenuation in thick biological tissues, caused by a combination of absorption and scattering, limits the penetration depth in multiphoton microscopy (MPM).
Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination
Journal of the Optical Society of America
We derive analytic expressions for the three-dimensional coherent transfer function (CTF) and coherent spread function (CSF) for coherent holographic image reconstruction by phase transfer (CHIRPT) microscopy with monochromatic and broadband illumination sources.
We explore the long wavelength limit of soliton self-frequency shift in silica-based fibers experimentally and using numerical simulation. We found that the longest wavelength soliton generated by soliton self-frequency shift is approximately 2500 nm.
Retrieving high-content gene-expression information while retaining three-dimensional (3D) positional anatomy at cellular resolution has been difficult, limiting integrative understanding of structure and function in complex biological tissues.
Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy
Spatial frequency modulated imaging (SPIFI) is a powerful imaging method that when used in conjunction with multiphoton contrast mechanisms has the potential to improve the spatial and temporal scales that can be explored within a single nonlinear optical microscope platform.
Biomedical Optics Express
Deep tissue multiphoton imaging requires high peak power to enhance signal and low average power to prevent thermal damage. Both goals can be advantageously achieved through laser repetition rate tuning instead of simply adjusting the average power.
The attenuation of excitation power reaching the focus is the main issue that limits the depth penetration of highresolution imaging of biological tissue. The attenuation is caused by a combination of tissue scattering and absorption.
We demonstrate three-photon microscopy (3PM) of mouse cerebellum at 1 mm depth by imaging both blood vessels and neurons. We compared 3PM and 2PM in the mouse cerebellum for imaging green and red fluorescence.
An approach combining signal detection theory and precise 3D reconstructions from serial section electron microscopy (3DEM) was used to investigate synaptic plasticity and information storage capacity at medial perforant path synapses in adult hippocampal dentate gyrus in vivo.
Balanced excitation and inhibition are required for high-capacity, noise-robust neuronal selectivity
Neurons and networks in the cerebral cortex must operate reliably despite multiple sources of noise. To evaluate the impact of noise, we determine the robustness of single-neuron stimulus selective responses and the robustness of attractor states of networks of neurons performing memory tasks.
Water deprivation produces a drive to seek and consume water. How neural activity creates this motivation remains poorly understood.
Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators
Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools.