An adaptive excitation source for multiphoton imaging

Conference on Lasers and Electro-Optics, OSA Technical Digest (online)
Abstract: We demonstrate an adaptive femtosecond laser source that improves the imaging speed by >10 times for multiphoton imaging of brain activity in awake mouse, achieving 30 frames/s, 734×734 µm field-of-view (FOV) at 700 µm depth. Authors: Bo Li, Mengran Wang, Chunyan Wu,

Interferometric spatial frequency modulation imaging

Optics Letters
Abstract: Interferometric spatial frequency modulation for imaging (I-SPIFI) is demonstrated for the first time, to our knowledge. Significantly, this imaging modality can be seamlessly combined with nonlinear SPIFI imaging and operates through single-element detection, making it compatible for use in scattering

Inferring single-trial neural population dynamics using sequential auto-encoders

Nature Methods
Abstract: Neuroscience is experiencing a revolution in which simultaneous recording of thousands of neurons is revealing population dynamics that are not apparent from single-neuron responses. This structure is typically extracted from data averaged across many trials, but deeper understanding requires studying

Three-photon imaging of mouse brain structure and function through the intact skull

Nature Methods
Abstract: Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and

How biological attention mechanisms improve task performance in a large-scale visual system model

eLife
Abstract: How does attentional modulation of neural activity enhance performance? Here we use a deep convolutional neural network as a large-scale model of the visual system to address this question. We model the feature similarity gain model of attention, in which

Comparing the effective attenuation lengths for long wavelength in vivo imaging of the mouse brain

Biomedical Optics Express
Abstract: Light attenuation in thick biological tissues, caused by a combination of absorption and scattering, limits the penetration depth in multiphoton microscopy (MPM). Both tissue scattering and absorption are dependent on wavelengths, which makes it essential to choose the excitation

Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination

Journal of the Optical Society of America
Abstract: We derive analytic expressions for the three-dimensional coherent transfer function (CTF) and coherent spread function (CSF) for coherent holographic image reconstruction by phase transfer (CHIRPT) microscopy with monochromatic and broadband illumination sources. The 3D CSF and CTF were used to

Investigation of the long wavelength limit of soliton self-frequency shift in a silica fiber

Optics Express
Abstract: We explore the long wavelength limit of soliton self-frequency shift in silica-based fibers experimentally and using numerical simulation. We found that the longest wavelength soliton generated by soliton self-frequency shift is approximately 2500 nm because the soliton loses its energy

Three-dimensional intact-tissue sequencing of single-cell transcriptional states

Science
Abstract: Retrieving high-content gene-expression information while retaining three-dimensional (3D) positional anatomy at cellular resolution has been difficult, limiting integrative understanding of structure and function in complex biological tissues. We developed and applied a technology for 3D intact-tissue RNA sequencing, termed

Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy

Applied Optics
Abstract: Spatial frequency modulated imaging (SPIFI) is a powerful imaging method that when used in conjunction with multiphoton contrast mechanisms has the potential to improve the spatial and temporal scales that can be explored within a single nonlinear optical microscope platform.

Fiber-based tunable repetition rate source for deep tissue two-photon fluorescence microscopy

Biomedical Optics Express
Abstract: Deep tissue multiphoton imaging requires high peak power to enhance signal and low average power to prevent thermal damage. Both goals can be advantageously achieved through laser repetition rate tuning instead of simply adjusting the average power. We show that

Comparison of excitation wavelengths for in vivo deep imaging of mouse brain

SPIE
Abstract: The attenuation of excitation power reaching the focus is the main issue that limits the depth penetration of highresolution imaging of biological tissue. The attenuation is caused by a combination of tissue scattering and absorption. Theoretical model of the effective

In vivo three-photon imaging of deep cerebellum

SPIE
Abstract: We demonstrate three-photon microscopy (3PM) of mouse cerebellum at 1 mm depth by imaging both blood vessels and neurons. We compared 3PM and 2PM in the mouse cerebellum for imaging green (using excitation sources at 1300 nm and 920 nm,

Long-term potentiation expands information content of hippocampal dentate gyrus synapses

PNAS
Abstract: An approach combining signal detection theory and precise 3D reconstructions from serial section electron microscopy (3DEM) was used to investigate synaptic plasticity and information storage capacity at medial perforant path synapses in adult hippocampal dentate gyrus in vivo. Induction of

Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy

Applied Optics
Abstract: Spatial frequency modulated imaging (SPIFI) is a powerful imaging method that when used in conjunction with multiphoton contrast mechanisms has the potential to improve the spatial and temporal scales that can be explored within a single nonlinear optical microscope platform.

Balanced excitation and inhibition are required for high-capacity, noise-robust neuronal selectivity

PNAS
Abstract: Neurons and networks in the cerebral cortex must operate reliably despite multiple sources of noise. To evaluate the impact of both input and output noise, we determine the robustness of single-neuron stimulus selective responses, as well as the robustness of

Thirst-associated preoptic neurons encode an aversive motivational drive

Science
Abstract: Water deprivation produces a drive to seek and consume water. How neural activity creates this motivation remains poorly understood.We used activity-dependent genetic labeling to characterize neurons activated by water deprivation in the hypothalamic median preoptic nucleus (MnPO). Single-cell transcriptional profiling

Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators

eLife
Abstract: Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise