Two-dimensional random access multiphoton spatial frequency modulated imaging

Optics Express
We explore the potential for the spatial frequency modulated imaging method when a spatial light modulator is used to program the excitation source, opening the door to a more versatile, random access imaging environment.

An adaptive excitation source for high-speed multiphoton microscopy

Nature Methods
Optical imaging is important for understanding brain function. However, established methods with high spatiotemporal resolution are limited by the potential for laser damage to living tissues.

Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates

We describe a subset of the many recent advances in circuit and cellular imaging tools in NHPs focusing here primarily on the research presented during the corresponding mini-symposium at the 2019 Society for Neuroscience annual meeting.

Non-invasive multiphoton imaging of neural structure and activity in Drosophila

We developed a non-invasive preparation for structural and functional imaging of the fly brain through the intact head cuticle. We first showed that the head cuticle transmits long-wavelength laser light with surprisingly high efficiencies.

Ensemble learning with 3D convolutional neural networks for functional connectome-based prediction

In this study, we critically evaluate the effect of brain parcellations on machine learning models applied to rs-fMRI data. Our experiments reveal that on average, models with stochastic parcellations consistently perform as well as models with widely used atlases at the same spatial scale.

Engineering a Less Artificial Intelligence

Despite enormous progress in machine learning, artificial neural networks still lag behind brains in their ability to generalize to new situations. Differences in generalization are caused by many defining features of a learning algorithm, such as network architecture and learning rule.

The BioLuminescent‐OptoGenetic in vivo response to coelenterazine is proportional, sensitive, and specific in neocortex

Journal of Neuroscience Research
BioLuminescent (BL) light production can modulate neural activity and behavior through co‐expressed OptoGenetic (OG) elements, an approach termed “BL‐OG.” Yet, the relationship between BL‐OG effects and bioluminescent photon emission has not been characterized in vivo.

The Visual Cortex in Context

Annual Review of Vision Science
In this article, we review the anatomical inputs and outputs to the mouse primary visual cortex, area V1. Our survey of data from the Allen Institute Mouse Connectivity project indicates that mouse V1 is highly interconnected with both cortical and subcortical brain areas.

Comparing the effective attenuation lengths for long wavelength in vivo imaging of the mouse brain

Biomedical Optics Express
we performed a systematic study of in vivo three-photon imaging at different excitation wavelengths, 1300 nm, 1450 nm, 1500 nm, 1550 nm, and 1700 nm, and quantified the tissue attenuation by calculating the effective attenuation length at each wavelength.