Miniaturized Devices for Bioluminescence Imaging in Freely Behaving Animals

Fluorescence miniature microscopy in vivo has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, photobleaching and non-homogeneous illumination artifacts.

An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals

Using a modular approach we have engineered Autonomous Molecular BioluminEscent Reporter (AMBER), an indicator of membrane potential.

jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Nature Methods
We report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

Deep three-photon imaging of the brain in intact adult zebrafish

Nature Methods
We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum.

Determining the Depth Limit of Bioluminescent Sources in Scattering Media

Here, we perform a theoretical study of the depth limits of bioluminescence microscopy and find that cellular resolution imaging should be possible at a depth of 5-10 mean free paths.

On the role of theory and modeling in neuroscience
This report is the summary of a discussion initiated at the conference Present and Future Theoretical Frameworks in Neuroscience, which we hope will contribute to a much-needed discussion in the neuroscientific community.

Cellular resolution imaging of neuronal activity across space and time in the mammalian brain

Current Opinion in Biomedical Engineering
This review focuses on recent advancements in optical methods that have pushed the boundaries for simultaneous population recordings at increasing volumes, distances, depths, and speeds.

Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain

We calculated and experimentally verified the excitation pulse energy to achieve the minimum photon count required for the detection of calcium transients in GCaMP6s-expressing neurons for 920 nm two-photon and 1320 nm three-photon excitation.

Simultaneous multi-dimensional spatial frequency modulation imaging

International Journal of Optomechatronics
Here, we demonstrate spatial frequency modulated imaging with enhanced resolution in multiple dimensions for the first time. This is achieved by incorporating multiple linear extended excitation sources oriented with axes at arbitrary angles with respect to each other.