Abstract: The action potential has long been understood to be the fundamental bit of information in brain, but how these spikes encode representations of stimuli and drive behavior remains unclear. Large-scale neuronal recordings with cellular and spike-time resolution spanning multiple brain regions are needed to capture relevant network dynamics that can be sparse and distributed across the population. This review focuses on recent advancements in optical methods that have pushed the boundaries for simultaneous population recordings at increasing volumes, distances, depths, and speeds. The integration of these technologies will be critical for overcoming fundamental limits in the pursuit of whole brain imaging in mammalian species.

Authors: Mitchell Clough, Jerry L.Chen