Abstract: Multicolor labeling of cardiac muscle with systemic AAVs. Three fluorescent proteins (mTurquoise2, mNeonGreen, and mRuby2) were separately packaged into AAV-PHP.S and systemically codelivered to a wild-type mouse at 3.3 × 1011vector genomes (vg) per virus (1 × 1012 vg total). Gene expression in the heart was evaluated 11 d later. Individual cardiomyocytes can be easily distinguished from one another.

Authors: Rosemary C. Challis, Sripriya Ravindra Kumar, Ken Y. Chan, Collin Challis, Keith Beadle, Min J. Jang, Hyun Min Kim, Pradeep S. Rajendran, John D. Tompkins, Kalyanam Shivkumar, Benjamin E. Deverman & Viviana Gradinaru